Journal: bioRxiv
Article Title: Identification of Antibiofilm Agents against Salmonella enterica from the Pathogen Box Compound Library
doi: 10.64898/2026.02.26.707623
Figure Lengend Snippet: Medium-throughput screen of the Pathogen Box library against S . Elisabethville LLD035X and S . Typhimurium ATCC 14028 biofilm formation (a-c). (a) Overview of the hit cascade used to prioritize compounds, resulting in two biofilm displaying none or minimal growth-inhibitory hits against S . Elisabethville LLD035X and one hit against S . Typhimurium ATCC 14028. MMV675968, MMV688179 and MMV687747 were initially picked as hits but were removed later because they could not reproduce antibiofilm activity. MMV687273 and MMV688371 were not available for further investigation. (b) & (c) Preliminary screening of 400 Pathogen Box compounds showing percentage biofilm inhibition versus percentage growth inhibition at 5 μM for S . Elisabethville LLD035X (b) and S . Typhimurium ATCC 14028 (c). The hit compounds are represented as blue and fall within the hit zone shaded as light blue. Compounds in Pathogen Box with known antibacterial effect against Gram-negative bacteria are represented in emerald green while the other non-hit compounds are shown in slate grey. Compounds above the horizontal line exhibit >30% biofilm inhibition, while those left of the vertical line show <10% growth inhibition. Two hits for S . Elisabethville and one hit for S . Typhimurium were identified.
Article Snippet: Compounds MMV687273 and MMV688978 did not meet hit criteria against inhibited S. Typhimurium ATCC 14028 because they only inhibited biofilm formation by 27.49% (p = 0.006) and 60.60% (p < 0.0001), respectively, but MMV688978 inhibited growth by 17.80% (p = 0.036).
Techniques: Activity Assay, Inhibition, Bacteria